restricted fragment meaning in English
限制酶切片段
Examples
- One 3 . 4 kb bamh i and pst i restricted fragment with intll positive was cloned from shigella flexneri 2a isolate c101 - 1 and sequenced . sequence analysis showed the fragment with a array of intll , atti site , two cassettes of blaoxa - 30 and aada1 , encoding oxacillinase and aminoglycoside adenylyltransferase respectively , and partial sequence of is1 , replacing the 3 " conserved region usually found in typical class 1 integron . the gene cassettes regions were examined in all these 30 isolates by pcr with a pair of primers annealing to the sequences of intll and is1 , and pcr products with same length were amplif
结果表明,此29株intll阳性志贺菌株有着相同的基因盒区,我们将之称为志贺菌非典型i类整合子( intll和inth一151同时阳性) ;福氏志贺菌中此非典型i类整合子总浙江大学博士学位论文检出率高达84 . 8 % ( 28 / 33 ) ,尤其是福氏2a和2b中均为100 % ( 13 / 13和9 / 9 ) :宋内志贺菌中则较低,为3 . 2 % ( l / 31 ) 。